Molecular Genetic Characterization of the Philadelphia Chromosome Detected in Reactor Personnel Highly Exposed to Radiation from the Chernobyl Accident
Title: Molecular Genetic Characterization of the Philadelphia Chromosome Detected in Reactor Personnel Highly Exposed to Radiation from the Chernobyl Accident
Author: Horst Zitzelsberger, Harald Hessel, Konstadinos Salassidis, Hans Mittermüller, Manfred Bauchinger
Reference: Cancer Genetics and Cytogenetics, Volume 104, Issue 2, 15 July 1998, Pages 86–93
DOI:http://dx.doi.org/10.1016/S0165-4608(97)00454-8
Keywords: chromosomes, liquidators
Abstract: Clonal del(22q) chromosome aberrations were coincidentally observed in highly exposed reactor personnel of the Chernobyl power plant accident in the course of retrospective biological dosimetry. These aberrant chromosomes were detected in PHA-stimulated cultures from peripheral blood after FPG staining and revealed a morphology similar to a Philadelphia chromosome. A rearrangement of the BCR gene on 22q11 could be confirmed in unstimulated peripheral blood by RFLP analysis from three of four del(22q) carrying cases. FISH analysis of the del(22q) carrying cases with BCR- and ABL-specific DNA probes additionally exhibited a BCR-ABL fusion in 5.2 to 9% of cells in unstimulated blood. Breakpoints within the BCR gene could be located either in the M-bcr or the m-bcr region and thus, a specific breakpoint region could not be detected in these four patients. Since typical clinical leukemic symptoms associated with the translocation (9;22)(q24;q11) could not be observed in these highly irradiated subjects (1.1 to 5.8 Gy), the role of this particular aberration in the development of a radiation-induced leukemia remains obscure.
ＵＲＬ：http://www.sciencedirect.com/science/article/pii/S0165460897004548
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