Comet assay on children’s leukocytes 8 years after the Chernobyl disaster

Title: Comet assay on children’s leukocytes 8 years after the Chernobyl disaster

Author: Frenzilli, G / Lori, A / Panasiuk, G / Ferdeghini, M / Barale, R

Reference: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 415 (1-2), p.151-158, Jul 1998

doi: 10.1016/S1383-5718(98)00060-6

Keywords: Single cell gel electrophoresis; Human leukocyte; DNA damage; Bleomycin; Adaptive response

Abstract…. The children come from the Gomel region, one of the areas most heavily radio-contaminated by the Chernobyl fallout. In addition, leukocytes were treated with a challenge dose of bleomycin (BLM, 1.5 μg/ml), to assess the presence of an adaptive response (AR) potentially resulting from chronic exposure to radionuclides. As controls, 13 children living in Pisa (Italy) were enrolled in the study. Children with thyroid cancer show higher (p<0.001) DNA damage than healthy ones.. …

URL: http://www.sciencedirect.com/science/article/pii/S1383571898000606

DNA damage evaluated by alkaline single cell gel electrophoresis (SCGE) in children of Chernobyl, 10 years after the disaster

Title: DNA damage evaluated by alkaline single cell gel electrophoresis (SCGE) in children of Chernobyl, 10 years after the disaster

Author: Frenzilli, G / Bosco, E / Antonelli, A / Panasiuk, G / Barale, R
Reference: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 491 (1-2), p.139-149, Apr 2001

doi: 10.1016/S1383-5718(01)00136-X

Keywords: Single cell gel electrophoresis; Human leukocytes; DNA damage; Bleomycin; Clastogenic factors

Abstract: Using the alkaline single cell gel electrophoresis (Comet) assay, the extent of DNA damage was evaluated in leukocytes of 43 Belarussian children (16 healthy and 27 affected by thyroid cancer). Thirty-nine healthy children from Pisa (Italy) were enrolled in the study as controls. In addition to basal levels of DNA damage, leukocytes were treated in vitro with bleomycin (BLM), a radiomimetic drug, to evaluate a possible adaptive response in different groups of children….

URL: http://www.sciencedirect.com/science/article/pii/S138357180100136X