Author: V.I. Khomenko
Reference: Український медичний часопис. (Ukirainian Medical magazine), 2008
Keywords: Chernobyl accident, autoimmune reactions, protein S-100, myelin basic protein
Abstract: In Chernobyl clean-up workers with cerebrovascular pathology who were irradiated with a range of doses from 30 to 94 cSv the reliable rise of HLA-DR+ lymphocytes activation under protein S-100 stimulation is revealed in comparison with those irradiated in doses up to 30 cSv. It probably testify to the alteration of the glial structures in the central nervous system (CNS) and blood-brain barrier (BBB), that increases under the higher doses irradiation. Glial and endothelial cells of brain vessels are elements of BBB. Conclusions about a probable increase of permeability of the BBB in Chernobyl clean-up workers with cerebrovascular pathology who were irradiated in a range of doses from 30 to 94 cSv, correspond to the results of other studies that have demonstrated an increase of the cerebrovascular discirculation with development of the white and grey brain matter atrophy with the threshold dose for occurrence of these changes 30 cSv. Delay of the HLA-DR+ lymphocyte activation that we observed below this threshold dose is probably connected with the influence of CD4-8+-Т cells on the lymphocyte activation.
URL: http://www.umj.com.ua/article/2215/stan-aktivacijnix-reakcij-limfocitiv-na-nejrospecifichni-antigeni-v-ulna-na-chaes-zalezhno-vid-doz-oprominennya
Title: Agricultural recovery of a formerly radioactive area: II. Systematic proteomic characterization of flax seed development in the remediated Chernobyl area
Author: Katarína Klubicová, Maksym Danchenko, Ludovit Skultety, Valentyna V. Berezhna, Andrea Hricová, Namik M. Rashydov, Martin Hajduch
Reference: Journal of Proteomics, Volume 74, Issue 8, 12 August 2011, Pages 1378-1384
DOI: http://dx.doi.org/10.1016/j.jprot.2011.02.029
Keywords: Linum usitatissimum L; Flax; Proteomics; Protein profiles; Remediation; Chernobyl; Radiation; Mass spectrometry
Abstract: Molecular characterization of crop plants grown in remediated, formerly radioactive, areas could establish a framework for future agricultural use of these areas. Recently, we have established a quantitative reference map for mature flax seed proteins (Linum usitatissimum L.) harvested from a remediated plot in Chernobyl town. Herein we describe results from our ongoing studies of this subject, and provide a proteomics-based characterization of developing flax seeds harvested from same field. A quantitative approach, based on 2-dimensional electrophoresis (2-DE) and tandem mass spectrometry, yielded expression profiles for 379 2-DE spots through seed development. Despite the paucity of genomic resources for flax, the identity for 102 proteins was reliably determined. These proteins were sorted into 11 metabolic functional classes. Proteins of unknown function comprise the largest group, and displayed a pattern of decreased abundance throughout seed development. Analysis of the composite expression profiles for metabolic protein classes revealed specific expression patterns during seed development. For example, there was an overall decrease in abundance of the glycolytic enzymes during seed development.
URL: http://www.sciencedirect.com/science/article/pii/S1874391911000856
