Micronucleus formation in lymphocytes of children from the vicinity of Chernobyl after 131I therapy

Title: Micronucleus formation in lymphocytes of children from the vicinity of Chernobyl after ¹³¹I therapy

Author: W.-U. Müller, S. Dietl, K. Wuttke, C. Reiners, J. Biko, E. Demidchik, C. Streffer

Reference: Radiation and Environmental Biophysics , May 2004, Volume 43, Issue 1, pp 7-13

doi: 10.1007/s00411-004-0233-z

Keywords : Lymphocytes, children, Iodine-131, thyroid tumours

Abstract: After the Chernobyl accident a statistically significant increase in the number of children with thyroid tumours was observed. In this study 166 children with and 75 without thyroid tumours were analysed for micronucleus formation in peripheral blood lymphocytes using the cytochalasin B approach. The following factors did not significantly affect micronucleus formation: gender, age at the time of the first ¹³¹I treatment, tumour stage, tumour type, or metastases; a statistically significant increase in the number of micronuclei, however, was observed for the residents of Gomel compared to other locations, such as Brest, Grodno, and Minsk. The children with tumours received ¹³¹I treatment after surgical resection of the tumours. This gave us the opportunity to systematically follow the effect of ¹³¹I on micronucleus formation. A marked increase was observed 5 days after the ¹³¹I treatment followed by a decrease within a 4–7 months interval up to the next application, but the pre-treatment levels were not achieved. Up to 10 therapy cycles were followed each including an analysis of micronucleus formation before and 5 days after ¹³¹I application. The response of the children was characterised by clear individual differences and the increase/decrease pattern of micronucleus frequencies induced by iodine-131 was correlated with a decrease/increase pattern in the number of lymphocytes.

ＵＲＬ: http://link.springer.com/article/10.1007/s00411-004-0233-z

Genomic instability in chidren born after the Chernobyl Nuclear Accident (in vivo and in vitro studies)

Title: Genomic instability in chidren born after the Chernobyl Nuclear Accident (in vivo and in vitro studies)

Author: A. V. Aghajanyan, I. I. Suskov

Reference: Russian Journal of Genetics , June 2010, Volume 46, Issue 6, pp 740-749

DOI: 10.1134/S1022795410060153

Keywords :

Abstract: Analysis of peripheral blood lymphocytes in children born after the accident at the Chernobyl Nuclear Power Plant in the period from 1987 to 2004 (permanent residents of territories contaminated with radionuclides, n = 92; and children of irradiated fathers-liquidators, n = 88) revealed increased levels of aberrant cells (ACs) and aberrations of the chromosomal type as compared to the control (P < 0.05). In three subgroups of children with different initial AC frequencies (children with high AC frequencies, ≥3%; children with medium AC frequencies, 2%; and children with low AC frequencies, ≤1%), the levels of aberrations of the chromosomal type are increased as compared to the control (P < 0.05). The levels of aberrant cells and chromosome aberrations (CAs) in the subgroup of children with ≥3% frequencies significantly differ from those in the subgroup of children with ≤1% AC frequencies. No dependence of the AC and CA frequencies on the year of birth after the Chernobyl accident was revealed. After fractional and single γ-irradiation (137Cs) of blood in vitro in the 10–30 cGy dose range, the average CA frequencies in the first and second mitoses increased in a similar way depending on the initial AC frequencies in the children and parents. All these results suggest an individual character of genomic instability induced by low radiation doses and its transgenerational phenomenon in the organisms of children.

ＵＲＬ：http://link.springer.com/article/10.1134/S1022795410060153

Chromosomal Aberrations in Blood Lymphocytes of the Residents of 30-km Chornobyl NPP Exclusion Zone

Title: Chromosomal Aberrations in Blood Lymphocytes of the Residents of 30-km Chornobyl NPP Exclusion Zone

Author: Larysa BEZDROBNA, Tetyana TSYGANOK, Olena ROMANOVA, Larysa TARASENKO, Volodymyr TRYSHYN and Ludmila KLIMKINA

Reference: Institute for Nuclear Research, National Academy of Science of Ukraine

Keywords: lymphocytes, Chernobyl exclusion zone, chromosomal aberrations, Ukraine

Abstract: A comparative сytogenetic examination of 33 self-settlers in the 30 km ChNPP Exclusion Zone and 31 residents in villages of Yahotyn district, Kyiv region was carried out in 1998-99.

ＵＲＬ：http://www.rri.kyoto-u.ac.jp/NSRG/reports/kr79/kr79pdf/Bezdrobna.pdf